| 特点 | |
|---|---|
| 特性 | 功能简要描述：常用于叶绿体；分离确定 适用物种：拟南芥、小麦、两耳草 方法：WB/IF | 优势 |
Rbcl（Rubisco large subunit）antibody, rabbit polyclonal
Background
Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably
homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies.
In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit
(15 kDa) is coded by a family of nuclear rbcS genes.
Immunogen: KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano) bacterial
RbcL protein sequences (ATCG00490).
Concentration: 1.0mg/ml
Expected/apparent MW: 55 kDa
Tested applications: Western blot (WB), IF
Species specificity: Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Zea mays
Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions
should be determined by the end use.)
Storage Buffer: PBS Buffer, pH7.4
Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2
years from date of receipt. Aliquot to avoid repeated freezing and thawing
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